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SOLVED: The seeding density of a C35 dish is 0.3 x 10^6 cells and it optimally holds 2 mL of media. A HeLa cell suspension was determined to contain 1.2 x 10^6
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Calculating original cell density from a serial dilution with plate count - YouTube
Cell Counting With a Hemocytometer: As Easy as 1, 2, 3